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Although they show slightly differential substrate preference, both isoforms display high specificity towards SGs and the organ-specific distribution of transcripts was positively correlated with the content of SGs in diploid and tetraploid C. Gentianaceae), belonging to the glycoside hydrolase 1 (GH1) family, was confirmed using in vitro assays with recombinant proteins, following their heterologous expression in E. Function of two -D-glucosidases (named CeBGlu1 and CeBGlu2) from centaury (Centaurium erythraea Rafn fam. These compounds are susceptible to hydrolysis mediated by enzymes B-glucosidases, and the resulting aglycones are subsequently directed towards different metabolic pathways in plants. Coordinated metabolic processes, such as biosynthesis and catabolism of SGs, ensure constitutive presence of these bitter tasting compounds in plant tissues, which plays a decisive role in the defence against pathogens and herbivores. Secoiridoid glucosides (SGs) are monoterpenoids derived from the iridoid cyclopentan-C-pyran skeleton with B-D glucose linked at C1 position. The online version of this article (doi:10.1007/s1110-z) contains supplementary material, which is available to authorized users. The other vectors, including the non-viral vectors pPZP5025 and pPZP3425, needed co-infiltration of the RNA-silencing inhibitor P19 to give good expression levels.

Our results show that pPZPTRBO, pJLTRBO and pEAQ-HT had comparable expression levels without co-infiltration of a RNA-silencing inhibitor. Here, a variety of expression vectors which were designed for transient and stable plant transformation, including pPZP3425, pPZP5025, pPZPTRBO, pJLTRBO, pEAQ-HT and pBY030-2R, was compared for the expression of green fluorescent protein and β-glucuronidase in Nicotiana benthamiana by Agrobacterium-mediated transient expression. Each of them is reported to be highly efficient, robust and cost-effective. Transient expression, on the other hand, can deliver recombinant proteins within a week, and many viral vectors have been constructed for that purpose. However, the production of stable transformed transgenic plants is a lengthy procedure. Production of recombinant proteins in plants is of increasing importance for practical applications.